Although Î²-galactooligosaccharides (GOS) are documented prebiotic compounds, little is known about their utilization by lactobacilli. Genome sequencing projects have proposed models for understanding prebiotic catabolism, but mechanisms remain to be elucidated for many prebiotic substrates. Probiotic microbes rely on their ability to survive in the gastrointestinal tract, adhere to mucosal surfaces, and metabolize available energy sources from dietary compounds, including prebiotics. Barrangou, Rodolphe Abou Hachem, Maher Lahtinen, Sampo Goh, Yong Jun Svensson, Birte Klaenhammer, Todd R. Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilusĪndersen, Joakim M. We demonstrate the role of LacT as a transcriptional activator of lac operon II in the presence of lactose and CcpA-independent regulation of the lac gene cluster in S. This suggests the involvement of another transcriptional regulator in the regulation of lac operon II, which is the BglG-family transcriptional antiterminator LacT.

This finding was further confirmed by Î²-galactosidase assays with PlacA- lacZ and PlacT- lacZ in the presence of either lactose or glucose as the sole carbon source in the medium. Deletion of lacR did not affect the expression of lac operon II, which encodes a lactose-specific phosphotransferase. This suggests a function of LacR as a transcriptional repressor of lac operon I, which encodes enzymes involved in the phosphorylated tagatose pathway in the absence of lactose or galactose. Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster revealed elevated expression of lac operon I even in the absence of lactose. LacR Is a Repressor of lacABCD and LacT Is an Activator of lacTFEG, Constituting the lac Gene Cluster in Streptococcus pneumoniaeĬomparison of the transcriptome of Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose revealed the elevated expression of various genes and operons, including the lac gene cluster, which is organized into two operons, i.e., lac operon I ( lacABCD) and lac operon II ( lacTFEG).

J141046 eve online